Modulation of oncogenic miRNA biogenesis using functionalized polyamines

Cathy Staedel (1) , Thi Phuong Anh Tran (2) , Julie Giraud (3) , Fabien Darfeuille (1) , Audrey Di Giorgio (2) , Nicolas J. Tourasse (1) , Franck Salin (4) , Philippe Uriac (5) , Maria Duca (2)

1 ARNA - Acides Nucléiques : Régulations Naturelle et Artificielle
2 ICN - Institut de Chimie de Nice
3 BaRITOn - Bordeaux Research in Translational Oncology
4 BioGeCo - Biodiversité, Gènes & Communautés
5 ISCR - Institut des Sciences Chimiques de Rennes

MicroRNAs are key factors in the regulation of gene expression and their deregulation has been directly linked to various pathologies such as cancer. The use of small molecules to tackle the overexpression of oncogenic miRNAs has proved its efficacy and holds the promise for therapeutic applications. Here we describe the screening of a 640-compound library and the identification of polyamine derivatives interfering with in vitro Dicer-mediated processing of the oncogenic miR-372 precursor (pre-miR-372). The most active inhibitor is a spermine-amidine conjugate that binds to the pre-miR-372 with a K-D of 0.15 mu M, and inhibits its in vitro processing with a IC50 of 1.06 mu M. The inhibition of miR-372 biogenesis was confirmed in gastric cancer cells overexpressing miR-372 and a specific inhibition of proliferation through de-repression of the tumor suppressor LATS2 protein, a miR-372 target, was observed. This compound modifies the expression of a small set of miRNAs and its selective biological activity has been confirmed in patient-derived ex vivo cultures of gastric carcinoma. Polyamine derivatives are promising starting materials for future studies about the inhibition of oncogenic miRNAs and, to the best of our knowledge, this is the first report about the application of functionalized polyamines as miRNAs interfering agents.

Domaines: Chimie thérapeutique

Cathy Staedel, Thi Phuong Anh Tran, Julie Giraud, Fabien Darfeuille, Audrey Di Giorgio, et al.. Modulation of oncogenic miRNA biogenesis using functionalized polyamines. Scientific Reports, 2018, 8 (1), pp.1667. ⟨10.1038/s41598-018-20053-5⟩. ⟨hal-01709540⟩