Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Estelle Lebègue (1) , Carole Farre (2) , Catherine Jose (3) , Joëlle Saulnier (2) , Florence Lagarde (2) , Yves Chevalier (4) , Carole Chaix (2) , Nicole Jaffrezic-Renault (2)

1 ISCR - Institut des Sciences Chimiques de Rennes
2 Interfaces & biosensors - Interfaces & biocapteurs
3 ISA - Institut des Sciences Analytiques
4 LAGEPP - Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique

Polydiacetylene (PDA) inserted in films or in vesicles has received increasing attention due to its property to undergo a blue-to-red colorimetric transition along with a change from non-fluorescent to fluorescent upon application of various stimuli. In this review paper, the principle for the detection of various microorganisms (bacteria, directly detected or detected through the emitted toxins or through their DNA, and viruses) and of antibacterial and antiviral peptides based on these responsive PDA vesicles are detailed. The analytical performances obtained, when vesicles are in suspension or immobilized, are given and compared to those of the responsive vesicles mainly based on the vesicle encapsulation method. Many future challenges are then discussed

Keywords: Virus; Peptides; Polydiacetylene; Bacteria; Vesicles; Biosensing; Toxins

Domains: Chemical Sciences; Theoretical and/or physical chemistry; Chemical Sciences; Polymers; Chemical Sciences; Material chemistry; Life Sciences [q-bio]; Pharmaceutical sciences; Galenic pharmacology

Estelle Lebègue, Carole Farre, Catherine Jose, Joëlle Saulnier, Florence Lagarde, et al.. Responsive Polydiacetylene Vesicles for Biosensing Microorganisms. Sensors, 2018, 18 (2), pp.599. ⟨10.3390/s18020599⟩. ⟨hal-01740158⟩