Isolation of Bioactive Compounds from Calicotome villosa Stems

J. Alhage (1) , H. Elbitar (2) , S. Taha (3) , J.-P. Guegan (1) , Z. Dassouki (2) , T. Vives (1) , T. Benvegnu (1)

1 ISCR - Institut des Sciences Chimiques de Rennes
2 LIU - Lebanese International University
3 LU / ULB - الجامعة اللبنانية [بيروت] = Lebanese University [Beirut] = Université libanaise [Beyrouth]

A phenylethanoid, two steroids, a flavone glucoside and a chalcone have been isolated for the first time from the stems of together with a previously isolated flavone glucoside. Their structures were determined by spectroscopic analyses (NMR, HRMS) as basalethanoïd B (), β-sitosterol and stigmasterol (), chrysine-7--β-d-glucopyranoside (), chrysine 7-((6''--acetyl)--β-d-glucopyranoside) () and calythropsin (). The crude extracts and the isolated compounds (except 4), were evaluated for their antioxidant, antimicrobial (against two Gram-positive bacterial strains: , , four Gram-negative bacterial strains: , , , and three yeasts: , , and ), hemolytic, antidiabetic, anti-inflammatory and cytotoxic activity. The crude extracts showed good ability to scavenge the free radical DPPH. Methanol stem extract followed by the dichloromethane stem extract showed moderate antimicrobial potency; furthermore, at 1 mg/mL the methanol extract showed an inhibition of growth comparable to nystatin. Dichloromethane, methanol, and aqueous extracts inhibited 98%, 90%, and 80% of HeLa cell proliferation at 2 mg/mL respectively. Weak hypoglycemic and hemolytic effects were exhibited by the crude extracts. Among all the tested compounds, compound showed remarkable hypoglycemic potential (93% at 0.1 mg/mL) followed by compound (90% at 0.3 mg/mL). Compound was the most effective in the DPPH scavenging assay (100% at 0.1 mg/mL) and cytotoxic assay on HeLa cells (99% and 90% after 24 and 48 h of treatment at 0.1 mg/mL, respectively). No anti-inflammatory effects were displayed by any of the crude extracts or the isolated compounds at any of the tested concentrations.

Mots clés: Antibacterial; Antidiabetic; Basalethano d B; Calythropsin; Calicotome villosa; Chrysine-7-O-β-D-glucopyranoside; Cytotoxicity; HeLa

Domaines: Chimie

J. Alhage, H. Elbitar, S. Taha, J.-P. Guegan, Z. Dassouki, et al.. Isolation of Bioactive Compounds from Calicotome villosa Stems. Molecules, 2018, 23 (4), pp.851. ⟨10.3390/molecules23040851⟩. ⟨hal-01774427⟩