Activation of T cells by dendritic cells exposed to a reference sensitizer Towards a promising model to assess the allergenic potential of chemicals

Cécile Huppert 1, 2 Christophe Paris 1, 3 Isabelle Langonné 2 Samuel Muller 2 Julianne Mathiot 2 Hakima Abdessadeq 2 François Gagnaire 2 Fabrice Battais 2 Isabelle Sponne 2
1 INGRES - Interactions Gènes-Risques environnementaux et Effets sur la Santé
2 TB - Département Toxicologie et biométrologie
3 Irset - Institut de recherche, santé, environnement et travail
Abstract : Background - Low molecular weight chemicals constitute one of the major causes of occupational allergies. European legislation on chemicals recommends limiting the use of in vivo models for assessing the sensitizing potential of chemicals, and encourages the development of integrated alternative methods. An in vitro mouse model of bone marrow-derived dendritic cells (BMDCs) that showed good accuracy (75%) and sensitivity (69%) has previously been developed to assess the sensitizing potential of chemicals. Objective - To assess the ability of BMDCs to activate T cells (TCs) in vitro. Methods - BMDCs pre-exposed to the reference sensitizer ammonium hexachloroplatinate (AHCP) were co-cultured with different subpopulations of TCs. TC activation was assessed by surface marker expression, proliferation, and cytokine release. Results - The results showed significant activation of TCs co-cultured with dendritic cells pre-exposed to AHCP as evaluated by CD124 expression, proliferation, and cytokine secretion. Moreover, the response of TCs appeared to be Th2-oriented. Naive TCs were shown to be involved in this response, and the removal of regulatory TCs did not improve the cell response. Conclusions - The BMDCs used in this previously developed model appear to have the ability to activate TCs, confirming that the BMDC model represents a reliable assay for assessing the sensitizing potential of chemicals.
Keywords : chemical allergens dendritic cells in vitro assay T cells
Document type :
Journal articles
Complete list of metadatas
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01795806
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Friday, May 18, 2018 - 5:07:24 PM
Last modification on : Thursday, July 11, 2019 - 1:00:33 AM

Identifiers

Collections

UNIV-RENNES1 | IRSET | UNIV-LORRAINE | BIOSIT | UR1-UFR-SVE | IRSET-10 | EHESP | UNIV-ANGERS | UNIV-RENNES | USPC

Citation

Cécile Huppert, Christophe Paris, Isabelle Langonné, Samuel Muller, Julianne Mathiot, et al.. Activation of T cells by dendritic cells exposed to a reference sensitizer Towards a promising model to assess the allergenic potential of chemicals. Contact Dermatitis, Wiley, 2018, 79 (2), pp.67-75. ⟨10.1111/cod.12991⟩. ⟨hal-01795806⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

101