High sensitivity of the Hematoflow™ solution for chronic myelomonocytic leukemia screening

Romain Vazquez (1) , Mikaël Roussel (2) , Bouchra Badaoui (1, 3) , Nicolas Freynet (1) , Sihem Tarfi (1, 3) , Eric Solary (4) , Dorothée Selimoglu-Buet (4) , Orianne Wagner-Ballon (5, 6)

1 AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP)
2 MICMAC - Microenvironment, Cell Differentiation, Immunology and Cancer
3 BCR - Biomécanique cellulaire et respiratoire
4 U1170 Inserm - Hématopoïèse normale et pathologique
5 Hôpital Henri Mondor
6 CHU Pontchaillou [Rennes]

Background: Accumulation of classical monocytes CD14++CD16– (also called MO1) ≥ 94% can accurately distinguish chronic myelomonocytic leukemia (CMML) from reactive monocytosis. The HematoFlow™ solution, able to quantify CD16 negative monocytes, could be a useful tool to manage monocytosis which remains a common issue in routine laboratories.Methods: Classical monocytes were quantified from 153 whole blood samples collected on EDTA using both flow cytometry methods, either MO1 percentage determination by the multiparameter assay previously published and regarded here as the reference method, or CD16 negative monocyte percentage determination by the means of HematoFlow™.Results: Both methods of classical monocyte percentage determination were highly and significantly correlated (r = 0.87, P < 0.0001). The HematoFlow™ solution leant toward an overestimation of the genuine classical monocyte percentages obtained by the reference method. Percentages of CD16 negative monocytes provided by HematoFlow were higher than 94% for all the 73 patients displaying classical monocytes MO1 found ≥94% by the reference method, indicating a sensitivity of 100%. Furthermore, the calculation of CD16 negative monocyte percentage can be easily computerized and integrated to the middleware.Conclusions: We propose a new application of the Hematoflow™ solution that can be used as a flag system for monocytosis management and CMML detection.

Keywords: classical monocyte accumulation; reactive monocytosis; flow cytometry; HematoflowTM; chronic myelomonocytic leukemia

Domains: Life Sciences [q-bio]; Cancer; Life Sciences [q-bio]; Human health and pathology; Hematology; Life Sciences [q-bio]; Bioengineering

Romain Vazquez, Mikaël Roussel, Bouchra Badaoui, Nicolas Freynet, Sihem Tarfi, et al.. High sensitivity of the Hematoflow™ solution for chronic myelomonocytic leukemia screening. Cytometry Part B: Clinical Cytometry, 2018, 94 (5), pp.658-661. ⟨10.1002/cyto.b.21600⟩. ⟨hal-01812481⟩