Long-term expression of melanopsin and channelrhodopsin causes no gross alterations in the dystrophic dog retina

B Ameline T Tshilenge M Weber Marine Biget 1 L Libeau C Caplette A Mendes-Madeira P Provost C Guihal P Picaud P. Moullier 2 V Pichard C Cronin C Isiegas
1 GEH - Génomique Environnementale et Humaine
2 Vecteurs viraux et transfert des gènes in vivo
Abstract : Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in a large animal model of retinal degeneration, the RPE65-deficient Briard dog model of Leber congenital amaurosis. Intravitreal injection of adeno-associated virus vectors expressing channelrhodopsin and melanopsin had no effect on retinal thickness over a 16-month period post injection. Our data support the safety of the optogenetic approach for the treatment of blindness.
Document type :
Journal articles
Complete list of metadatas
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01846678
Contributor : Valerie Briand <>
Submitted on : Sunday, July 22, 2018 - 5:07:32 PM
Last modification on : Friday, November 30, 2018 - 10:16:08 AM

Identifiers

Collections

UNIV-RENNES1 | UNIV-RENNES

Citation

B Ameline, T Tshilenge, M Weber, Marine Biget, L Libeau, et al.. Long-term expression of melanopsin and channelrhodopsin causes no gross alterations in the dystrophic dog retina. Gene Therapy, Nature Publishing Group, 2017, 24 (11), pp.735-741. ⟨10.1038/gt.2017.63⟩. ⟨hal-01846678⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

27