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Journal articles
Long-term expression of melanopsin and channelrhodopsin causes no gross alterations in the dystrophic dog retina
B Ameline
T Tshilenge
M Weber
Marine Biget
1
L Libeau
C Caplette
A Mendes-Madeira
P Provost
C Guihal
P Picaud
P. Moullier
2
V Pichard
C Cronin
C Isiegas
1
GEH - Génomique Environnementale et Humaine (Plateforme 'Génomique Environnementale et Humaine', Observatoire des Sciences de l'Univers de Rennes, Université de Rennes 1. - France)
- UR1 - Université de Rennes 1 (2 rue du Thabor - CS 46510 - 35065 Rennes cedex - France)
- UNIV-RENNES - Université de Rennes (France)
2
Vecteurs viraux et transfert des gènes in vivo (30 Avenue Jean Monnet, 44035 Nantes - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : ERM0105 (101, rue de Tolbiac, 75013 Paris - France)
Abstract : Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in a large animal model of retinal degeneration, the RPE65-deficient Briard dog model of Leber congenital amaurosis. Intravitreal injection of adeno-associated virus vectors expressing channelrhodopsin and melanopsin had no effect on retinal thickness over a 16-month period post injection. Our data support the safety of the optogenetic approach for the treatment of blindness.
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-01846678
Contributor : Valerie Briand <>
Submitted on : Sunday, July 22, 2018 - 5:07:32 PM
Last modification on : Friday, November 30, 2018 - 10:16:08 AM
Contributor : Valerie Briand <>
Submitted on : Sunday, July 22, 2018 - 5:07:32 PM
Last modification on : Friday, November 30, 2018 - 10:16:08 AM
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- HAL Id : hal-01846678, version 1
- DOI : 10.1038/gt.2017.63
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B Ameline, T Tshilenge, M Weber, Marine Biget, L Libeau, et al.. Long-term expression of melanopsin and channelrhodopsin causes no gross alterations in the dystrophic dog retina. Gene Therapy, Nature Publishing Group, 2017, 24 (11), pp.735-741. ⟨10.1038/gt.2017.63⟩. ⟨hal-01846678⟩
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