Skip to Main content Skip to Navigation
Journal articles

Chronic refined low-fat diet consumption reduces cholecystokinin satiation in rats

Abstract : Reduced ability of cholecystokinin (CCK) to induce satiation contributes to hyperphagia and weight gain in high-fat/high-sucrose (HF/HS) diet-induced obesity, and has been linked to altered gut microbiota. Rodent models of obesity use chow or low-fat (LF) diets as control diets; the latter has been shown to alter gut microbiota and metabolome. We aimed to determine whether LF-diet consumption impacts CCK satiation in rats and if so, whether this is prevented by addition of inulin to LF diet. Rats (n = 40) were fed, for 8 weeks, a chow diet (chow) or low-fat (10%) or high-fat/high-sucrose (45 and 17%, respectively) refined diets with either 10% cellulose (LF and HF/HS) or 10% inulin (LF-I and HF/HS-I). Caecal metabolome was assessed by 1H-NMR-based metabolomics. CCK satiation was evaluated by measuring the suppression of food intake after intraperitoneal CCK injection (1 or 3 µg/kg). LF-diet consumption altered the caecal metabolome, reduced caecal weight, and increased IAP activity, compared to chow. CCK-induced inhibition of food intake was abolished in LF diet-fed rats compared to chow-fed rats, while HF/HS diet-fed rats responded only to the highest CCK dose. Inulin substitution ameliorated caecal atrophy, reduced IAP activity, and modulated caecal metabolome, but did not improve CCK-induced satiety in either LF- or HF/HS-fed rats. CCK signaling is impaired by LF-diet consumption, highlighting that caution must be taken when using LF diet until a more suitable refined control diet is identified.
Complete list of metadatas

Cited literature [46 references]  Display  Hide  Download

https://hal-univ-rennes1.archives-ouvertes.fr/hal-01862581
Contributor : Laurent Jonchère <>
Submitted on : Friday, September 14, 2018 - 10:47:57 AM
Last modification on : Thursday, October 15, 2020 - 3:06:02 PM
Long-term archiving on: : Saturday, December 15, 2018 - 4:03:35 PM

File

Guerville et al_Chronic refine...
Files produced by the author(s)

Identifiers

Citation

Mathilde Guerville, M Kristina Hamilton, Charlotte C Ronveaux, Sandrine Ellero-Simatos, Helen E Raybould, et al.. Chronic refined low-fat diet consumption reduces cholecystokinin satiation in rats. European Journal of Nutrition, Springer Verlag, 2019, 58 (6), pp.2497-2510. ⟨10.1007/s00394-018-1802-2⟩. ⟨hal-01862581⟩

Share

Metrics

Record views

305

Files downloads

430