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, Accepted Article

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, HeLa cells were transfected with FLAG-ATF6 plasmid alone (0.5?g) or along with (0.25?g) HA-EDEM1 (0.5?g) or HA-EDEM1-N198I (0.5?g). 24 hours later the cells were treated with 1mM DTT for 0, 0.5, 1, 2 and 3 hours and then lysed in RIPA buffer. The protein lysates were immunoblotted for FLAG and EDEM1. The western blot shown here is representative of three independent experiments. (D) Protein quantification using the ImageJ software for the experiment described in (C) with 100% being the ATF6-p90 protein level at 0h time-point in each case. (E) Protein quantification as in (D) for ATF6-p50 only from cells co-expressing FLAG-ATF6 with HA-EDEM1 or HAEDEM1-N198I. The data are representative of three or more independent experiments and the values shown are the mean ±SEM of the respective experiments (**p<0.01) (F) Protein quantification of ATF6-p50 from Figure 5E normalized to the HA-EDEM1 or HA-EDEM1N198I expression levels (data are representative of three or more independent experiments and the values shown are the mean ±SEM of the respective experiments). (G) HeLa cells were transfected with FLAG-ATF6 (0.25?g) and HA-EDEM1 (0.5?g) or HA-EDEM1-N198I (0.5?g), Accepted Article . Figure 5. Comparison of the impact of EDEM1 wild type and N198I mutant on ATF6 activation. (A) Schematic representation of the EDEM1 protein and its N-linked glycosylation sites. (B) Expression of 0.1, 0.5, 1 and 2 ?g of the HA-EDEM1 and HAEDEM1-N198I plasmids in HeLa cells. (C)

, A) HeLa cells were transfected with FLAG-ATF6 plasmid (0.5?g) alone or in combination with HA-EDEM1 or HA-EDEM1-N198I plasmids at a series of different doses: 0.5, 1, 2, 5, and 10?g. The cells were lysed 24 hours post-transfection in RIPA buffer. Equal amounts of protein lysates were immunoblotted for ATF6, EDEM1 and Actin. (B) Protein quantification of the experiment described in (A) for the ATF6-p90 form. A statistical difference between the two EDEM1 forms was shown only for the dose of 0.5?g, represented in the bar graph here. The ATF6-p90 protein level at the "Flag-ATF6" alone condition is regarded as 100%. (C) HeLa cells were transfected with FLAG-ATF6 plasmid (0.5?g) alone or in combination with HA-EDEM1 or HA-EDEM1-N198I plasmids (0.5?g). 24 hours later, the cells were either left untreated (0h time-point) or treated with cycloheximide (CHX) at a final concentration of 5?g/ml for 1, 2, 3, and 6 hours. Equal amounts of protein lysates were immunoblotted for ATF6, EDEM1 and Actin

, At the same time whole cell lysates (WCL) were kept by lysing the cells in RIPA buffer. The samples of the secreted proteins along with their corresponding WCL were immunoblotted for ?1-antitrypsin, EDEM1 and VCP as the loading control for the WCL where indicated.Protein quantification of EDEM1 levels presented in Figure 7G using ImageJ software for HeLa (B) and HuH7 cells (C). The bars are representative of three