Organic chemicals from diesel exhaust particles affects intracellular calcium, inflammation and β-adrenoceptors in endothelial cells

Abstract : Exposure to diesel exhaust particles (DEP) may contribute to endothelial dysfunction and cardiovascular disease. DEP, extractable organic material from DEP (DEP-EOM) and certain PAHs seem to trigger [Ca] increase as well as inflammation via GPCRs like βARs and PAR-2. In the present study we explored the involvement of βARs and PAR-2 in effects of DEP-EOM on [Ca] and expression of inflammation-associated genes in the endothelial cell-line HMEC-1. We exposed the human microvascular endothelial cell line HMEC-1 to DEP-EOM fractionated by sequential extraction with solvents of increasing polarity n-hexane (n-Hex-EOM), dichloromethane (DCM-EOM), methanol (Methanol-EOM) and water (Water-EOM). While Methanol-EOM and Water-EOM had no marked effects, n-Hex-EOM and DCM-EOM enhanced [Ca] (2-3 times baseline) and expression of inflammation-associated genes (IL-1α, IL-1β, COX-2 and CXCL8; 2-15 times baseline) in HMEC-1. The expression of βARs (60-80% of baseline) and βAR-inhibitor carazolol suppressed the increase in [Ca] induced by both n-Hex- and DCM-EOM. Carazolol as well as the Ca-channel inhibitor SKF-96365 reduced the DCM-EOM-induced pro-inflammatory gene-expression. Overexpression of βARs increased DCM-EOM-induced [Ca] responses in HEK293 cells, while βAR-overexpression suppressed [Ca] responses from n-Hex-EOM. Furthermore, the PAR-2-inhibitor ENMD-1068 attenuated [Ca] responses to DCM-EOM, but not n-Hex-EOM in HMEC-1. The results suggest that βAR and PAR-2 are partially involved in effects of complex mixtures of chemicals extracted from DEP on calcium signalling and inflammation-associated genes in the HMEC-1 endothelial cell-line.
Liste complète des métadonnées

https://hal-univ-rennes1.archives-ouvertes.fr/hal-01976471
Contributor : Laurent Jonchère <>
Submitted on : Friday, January 25, 2019 - 1:09:30 PM
Last modification on : Thursday, February 7, 2019 - 5:11:57 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2019-05-29

Please log in to resquest access to the document

Identifiers

Citation

Bendik C Brinchmann, Eric Le Ferrec, Normand Podechard, Dominique Lagadic-Gossmann, Jørn A Holme, et al.. Organic chemicals from diesel exhaust particles affects intracellular calcium, inflammation and β-adrenoceptors in endothelial cells. Toxicology Letters, Elsevier, 2019, 302, pp.18-27. ⟨10.1016/j.toxlet.2018.11.009⟩. ⟨hal-01976471⟩

Share

Metrics

Record views

25