Skip to Main content Skip to Navigation
Journal articles

Efferocytosis capacities of blood monocyte‐derived macrophages in systemic sclerosis

Abstract : A defect in the apoptotic cell clearance (efferocytosis) by phagocytic cells may participate in autoimmunity and chronic inflammation. The mechanisms leading to the emergence of autoimmunity in systemic sclerosis (SSc) are still to be determined. In this study, the efferocytosis capacities of blood monocyte‐derived macrophages (MDM) from patients with SSc were evaluated. Blood monocytes obtained from patients with SSc and healthy donors (HD) were differentiated in vitro into macrophages. The capacities of MDM to engulf CFSE+ apoptotic Jurkat human T lymphocytes were compared between SSc MDM and HD using flow cytometry. The expression of classical engulfing receptors in SSc MDM and HD MDM was also evaluated and their involvement in the modulation of efferocytosis was confirmed using a siRNA approach. The mean phagocytic index (PI) reflecting efferocytosis capacities of SSc MDM (PI = 19.3 ± 3.0; n = 21) was significantly decreased in comparison with the PI of HD MDM (PI = 35.9 ± 3.0; n = 31; P < 0.001). In comparison with HD, SSc MDM exhibited a downregulated expression of scavenger receptor (SR)‐B1, SR‐A1 and integrin β5 (ITGβ5). In HD MDM, the extinction of these receptors was followed by a reduction of efferocytosis only for the repression of ITGβ5, suggesting a possible selective role of this integrin in the impaired efferocytosis observed in SSc. As efferocytosis may be at the crossroads of inflammation, autoimmunity and fibrosis, in showing impaired efferocytosis capacities of blood MDM in SSc, our study offers new pathogenesis considerations for the involvement of macrophages in the autoimmune processes driving this disorder.
Document type :
Journal articles
Complete list of metadatas

Cited literature [30 references]  Display  Hide  Download
Contributor : Laurent Jonchère <>
Submitted on : Wednesday, January 23, 2019 - 10:19:08 AM
Last modification on : Thursday, January 14, 2021 - 11:17:35 AM


Efferocytosis capacities of bl...
Files produced by the author(s)



Alice Ballerie, Alain Lescoat, Yu Augagneur, Marie Lelong, Claudie Morzadec, et al.. Efferocytosis capacities of blood monocyte‐derived macrophages in systemic sclerosis. Immunology and Cell Biology, Nature Publishing Group, 2019, 97 (3), pp.340-347. ⟨10.1111/imcb.12217⟩. ⟨hal-01978064⟩



Record views


Files downloads