Is it worth having an adequate energetic state to detoxify a pesticide?
Résumé
The use of pesticides for pest control impacts on ecosystems, affecting directly or indirectly non-target organisms such as spiders. How they respond in the face of xenobiotics depends, among other factors, on their energetic state, which is a reflection of their metabolic state. In the present work we assess the energetic state of Polybetes pythagoricus in different metabolic situations (females before and after vitellogenesis, juvenile, and adult males), and how cypermethrin affects mortality, the enzyme activity of the antioxidant system, and lipid peroxidation. As a result, it was observed that the calories provided by both glycogen and lipids in females before and after vitellogenesis were 38.1 and 22.4 cal/gr respectively. In the case of malesand juveniles, the values were of 33.4 cal/gr and 56.1 cal/gr respectively. The triacylglyceride/free fatty acid relation determined that juveniles have higher lipid dynamics. The LD50 for females before and after vitellogenesis were 969 and 1108 ngr/gr, and was 1060 ngr/gr and 103 ngr/gr for males and juveniles respectively. In exposed adult spiders, we observed an increase of 0.8 to 5.5 times in superoxide-dismutase activity compared to control. The catalase activity of males saw no significant increase while an increase in females was observed. Lipid peroxidation showed an increase in adults of 0.5 times. Although juveniles had a high caloric content, no increase was observed in superoxide-dismutase activity and catalase, coinciding with the high increment observed in the lipid peroxidation (14 times) produced by the reactive oxygen species.