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Journal articles
Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time- resolved X-ray scattering
Abstract : Phytochromes are photoreceptor proteins that transmit a light signal from a photosensory region to an output domain. Photoconversion involves protein conformational changes whose nature is not fully understood. Here, we use time-resolved X-ray scattering and optical spectroscopy to study the kinetics of structural changes in a full-length cyanobacterial phytochrome and in a truncated form with no output domain. X-ray and spectroscopic signals on the mu s/ms timescale are largely independent of the presence of the output domain. On longer time-scales, large differences between the full-length and truncated proteins indicate the timeframe during which the structural transition is transmitted from the photosensory region to the output domain and represent a large quaternary motion. The suggested independence of the photosensory-region dynamics on the mu s/ms timescale defines a time window in which the photoreaction can be characterized (e.g. for optogenetic design) independently of the nature of the engineered output domain.
Cited literature [22 references]
https://hal-univ-rennes1.archives-ouvertes.fr/hal-02086213
Contributor : Laurent Jonchère <>
Submitted on : Tuesday, September 22, 2020 - 5:14:47 PM
Last modification on : Tuesday, January 12, 2021 - 4:41:53 PM
Long-term archiving on: : Wednesday, December 23, 2020 - 6:28:01 PM
Contributor : Laurent Jonchère <>
Submitted on : Tuesday, September 22, 2020 - 5:14:47 PM
Last modification on : Tuesday, January 12, 2021 - 4:41:53 PM
Long-term archiving on: : Wednesday, December 23, 2020 - 6:28:01 PM
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