Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time- resolved X-ray scattering

Derren J. Heyes 1 Samantha J. O. Hardman 1 Martin N. Pedersen 2 Joyce Woodhouse 3 Eugenio de la Mora 3 Michael Wulff 2 Martin Weik 3 Marco Cammarata 4 Nigel S. Scrutton 1 Giorgio Schirò 3
1 University of Manchester [Manchester]
2 ESRF - European Synchrotron Radiation Facility
3 IBS - UMR 5075 - Institut de biologie structurale
4 IPR - Institut de Physique de Rennes
Abstract : Phytochromes are photoreceptor proteins that transmit a light signal from a photosensory region to an output domain. Photoconversion involves protein conformational changes whose nature is not fully understood. Here, we use time-resolved X-ray scattering and optical spectroscopy to study the kinetics of structural changes in a full-length cyanobacterial phytochrome and in a truncated form with no output domain. X-ray and spectroscopic signals on the mu s/ms timescale are largely independent of the presence of the output domain. On longer time-scales, large differences between the full-length and truncated proteins indicate the timeframe during which the structural transition is transmitted from the photosensory region to the output domain and represent a large quaternary motion. The suggested independence of the photosensory-region dynamics on the mu s/ms timescale defines a time window in which the photoreaction can be characterized (e.g. for optogenetic design) independently of the nature of the engineered output domain.
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Journal articles
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https://hal-univ-rennes1.archives-ouvertes.fr/hal-02086213
Contributor : Laurent Jonchère <>
Submitted on : Monday, April 1, 2019 - 11:15:27 AM
Last modification on : Wednesday, June 26, 2019 - 10:03:14 AM

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Derren J. Heyes, Samantha J. O. Hardman, Martin N. Pedersen, Joyce Woodhouse, Eugenio de la Mora, et al.. Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time- resolved X-ray scattering. Communications Biology, 2019, 2 (1), pp.1. ⟨10.1038/s42003-018-0242-0⟩. ⟨hal-02086213⟩

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