Quantitative resistance to clubroot deconvoluted into QTL-specific metabolic modules

Abstract : Plant disease resistance is often under quantitative genetic control. Thus in a given interaction, plant cellular responses to infection are influenced by resistance or susceptibility alleles at different loci. In this work, a genetic-linkage analysis was used to address the complexity of metabolic responses of Brassicanapus roots to the infection by Plasmodiophorabrassicae. Metabolome profiling and pathogen quantification in a segregating progeny allowed a comparative mapping of Quantitative Trait Loci (QTL) involved in resistance and in metabolic adjustments. Distinct metabolic modules were associated to each resistance QTL, suggesting the involvement of different underlying cellular mechanisms. This approach highlighted the possible role of gluconasturtiin and of two unknown metabolites in the resistance conferred by two QTL on chromosomes C03 and C09, respectively. Only two susceptibility biomarkers (glycine and glutathione) were simultaneously linked to the three main resistance QTL, suggesting the central role of those compounds in the interaction. By contrast, several genotype-specific metabolic responses to infection were genetically unconnected to resistance or susceptibility. Likewise, variations of root sugar profiles, which might have influenced pathogen nutrition, were found unrelated to resistance QTL. This work illustrates how genetical metabolomics can help to understand plant stress responses and their possible links with disease.
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Geoffrey Wagner, Anne Laperche, Christine Lariagon, Nathalie Marnet, David Renault, et al.. Quantitative resistance to clubroot deconvoluted into QTL-specific metabolic modules. Journal of Experimental Botany, Oxford University Press (OUP), inPress, ⟨10.1093/jxb/erz265⟩. ⟨hal-02150226⟩

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