Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast

Balveer Singh 1 Kamlesh K. Bisht Udita Upadhyay Avinash Chandra Kushwaha Jagpreet Singh Nanda 2 Suchita Srivastava Jai Kumar Saini Amar J. S. Klar 3 Jagmohan Singh
1 IGDR - Institut de Génétique et Développement de Rennes
2 Case Western Reserve University [Cleveland]
3 NIH - National Cancer Institute
Abstract : The developmental asymmetry of fission yeast daughter cells derives from inheriting older Watson' versus older Crick' DNA strand from the parental cell, strands that are complementary but not identical with each other. A novel DNA strand-specific imprint', installed during DNA replication at the mating-type locus (mat1), imparts competence for cell type inter-conversion to one of the two chromosome replicas. The catalytic subunit of DNA Polymerase (Pol) has been implicated in the imprinting process. Based on its known biochemical function, Pol might install the mat1 imprint during lagging strand synthesis. The nature of the imprint is not clear it is either a nick or a ribonucleotide insertion. Our investigations do not support a direct role of Pol in nicking through putative endonuclease domains but confirm its indirect role in installing an alkali-labile moiety as the imprint. While ruling out the role of the primase subunit of Pol holoenzyme, we find that mutations in the Pol-recruitment and putative primase homology domain in Mcm10/Cdc23 abrogate the ribonucleotide imprint formation. These results, while confirming the ribonucleotide nature of the imprint suggest the possibility of a direct role of Mcm10/Cdc23 in installing it in cooperation with Pol and Swi1.
Document type :
Journal articles
Complete list of metadatas

Cited literature [53 references]  Display  Hide  Download
https://hal-univ-rennes1.archives-ouvertes.fr/hal-02153242
Contributor : Laurent Jonchère <>
Submitted on : Wednesday, September 4, 2019 - 10:39:21 AM
Last modification on : Wednesday, September 4, 2019 - 2:07:45 PM

File

gkz092.pdf
Publisher files allowed on an open archive

Identifiers

Collections

UNIV-RENNES1 | UR1-UFR-SVE | BIOSIT | IGDR | UNIV-RENNES

Citation

Balveer Singh, Kamlesh K. Bisht, Udita Upadhyay, Avinash Chandra Kushwaha, Jagpreet Singh Nanda, et al.. Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast. Nucleic Acids Research, Oxford University Press, 2019, 47 (7), pp.3422-3433. ⟨10.1093/nar/gkz092⟩. ⟨hal-02153242⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

16

Files downloads

15