Skip to Main content Skip to Navigation
Journal articles

Staphylococcus aureus induces DNA damage in host cell

Abstract : Staphylococcus aureus causes serious medical problems in human and animals. Here we show thatS. aureus can compromise host genomic integrity as indicated by bacteria-induced histone H2AXphosphorylation, a marker of DNA double strand breaks (DSBs), in human cervix cancer HeLa andosteoblast-like MG-63 cells. This DNA damage is mediated by alpha phenol-soluble modulins (PSMα1–4),while a specific class of lipoproteins (Lpls), encoded on a pathogenicity island in S. aureus, dampens theH2AX phosphorylation thus counteracting the DNA damage. This DNA damage is mediated by reactiveoxygen species (ROS), which promotes oxidation of guanine forming 7,8-dihydro-8-oxoguanine (8-oxoG). DNA damage is followed by the induction of DNA repair that involves the ATM kinase-signalingpathway. An examination of S. aureus strains, isolated from the same patient during acute initial andrecurrent bone and joint infections (BJI), showed that recurrent strains produce lower amounts of Lpls,induce stronger DNA-damage and prompt the G2/M transition delay to a greater extent that suggest aninvolvement of these mechanisms in adaptive processes of bacteria during chronicization. Our findingsredefine our understanding of mechanisms of S. aureus-host interaction and suggest that the balancebetween the levels of PSMα and Lpls expression impacts the persistence of the infection.
Complete list of metadata

Cited literature [67 references]  Display  Hide  Download
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Wednesday, June 12, 2019 - 8:43:01 PM
Last modification on : Saturday, September 24, 2022 - 3:10:05 PM


Explicit agreement for this submission


Distributed under a Creative Commons Attribution 4.0 International License



Martine Deplanche, Nassim Mouhali, Minh-Thu Nguyen, Chantal Cauty, Frederic Ezan, et al.. Staphylococcus aureus induces DNA damage in host cell. Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.7694. ⟨10.1038/s41598-019-44213-3⟩. ⟨hal-02153435⟩



Record views


Files downloads