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Journal articles
A V0-ATPase-dependent apical trafficking pathway maintains the polarity of the intestinal absorptive membrane
Abstract : Intestine function relies on the strong polarity of intestinal epithelial cells and the array of microvilli forming a brush border at their luminal pole. Combining a genetic RNA interference (RNAi) screen with super-resolution imaging in the intestine, we found that the V0 sector of the vacuolar ATPase (V0-ATPase) controls a late apical trafficking step, involving Ras-related protein 11 (RAB-11) endosomes and the -ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) synaptosome-associated protein 29 (SNAP-29), and is necessary to maintain the polarized localization of both apical polarity modules and brush border proteins. We show that the V0-ATPase pathway also genetically interacts with glycosphingolipids and clathrin in enterocyte polarity maintenance. Finally, we demonstrate that silencing of the V0-ATPase fully recapitulates the severe structural, polarity and trafficking defects observed in enterocytes from individuals with microvillus inclusion disease (MVID) and use this new MVID model to follow the dynamics of microvillus inclusions. Thus, we describe a new function for V0-ATPase in apical trafficking and epithelial polarity maintenance and the promising use of the intestine as an model to better understand the molecular mechanisms of rare genetic enteropathies.
Cited literature [61 references]
https://hal-univ-rennes1.archives-ouvertes.fr/hal-02153480
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Monday, July 1, 2019 - 4:22:12 PM
Last modification on : Friday, February 19, 2021 - 2:51:48 PM
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Monday, July 1, 2019 - 4:22:12 PM
Last modification on : Friday, February 19, 2021 - 2:51:48 PM
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Bidaud-Meynard-2019-A V0-ATPas...
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