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, Histogram shows the measurement of microvilli length from TEM images (n=5 microvilli measured/worm), V0/vha-1(RNAi) structural defects appear after 72h silencing
, V0-or V1-ATPase subunits silencing affects C. elegans development. Histogram shows the measurement of the worms' diameter from TEM images
, V0/vha-1 silencing often induced a detachment of the PM from the terminal web (C) as well as (D-E) increased the length of cell junctions (CeAJ). (E) shows the measurement of the electron-dense cell-cell junction length on TEM pictures
, V0/vha-1 depleted worms accumulate cell and/or bacterial debris in the intestinal lumen
, V0/vha-1 depleted N2 worms, contrary to control or V1/vha-8-depleted worms, are devoid of yolk storage granules (arrows). L, lumen; mv, microvilli; tw
,
,
, CeAJ, C. elegans adherens junctions
, In micrographs, worms are at the L4/young adult (72h RNAi) or adult
, Histograms show the mean ± SEM, dots represent individual worms and the total number of worms is indicated in brackets. n.s. non-significant, *p<0,05, ****p<0,0001