Myelinosome-like vesicles in human seminal plasma A cryo-electron microscopy study

Abstract : Seminal plasma is particularly rich in extracellular vesicles. Myelinosomes are membranous organelles described throughout the seminiferous epithelium of the testis but never reported in semen. Our aim was to determine the presence of myelinosomes in human seminal plasma. Transmission electron microscopy and cryo electron microscopy analysis of standard myelinosome preparation from TM4 Sertoli cells and human seminal plasma samples. We have specified by cryo-EM the morphological aspect of "standard" myelinosomes isolated from the culture media of TM4 Sertoli cells. Vesicles with the same morphological appearance were revealed in human seminal plasma samples. Human seminal plasma contains a population of large EV (average diameter 200 nm) whose morphological appearance resemble those of myelinosomes. Defining the specific biomarkers and functionalities of myelinosome in human seminal plasma are the concerns to be addressed in our further research.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-rennes1.archives-ouvertes.fr/hal-02304994
Contributor : Laurent Jonchère <>
Submitted on : Thursday, October 3, 2019 - 4:15:41 PM
Last modification on : Monday, October 21, 2019 - 4:10:34 PM

Identifiers

Citation

M Yefimova, E Bere, a S Neyroud, Bernard Jégou, N Bourmeyster, et al.. Myelinosome-like vesicles in human seminal plasma A cryo-electron microscopy study. Cryobiology, 2019, ⟨10.1016/j.cryobiol.2019.09.009⟩. ⟨hal-02304994⟩

Share

Metrics

Record views

22