In vitro study of human endothelial progenitor cells behaviour on nitrided Ni-free Ti-27Nb alloy
Résumé
A superelastic Ni-free Ti-27Nb alloy has been synthesized and gas nitrided at high temperature to investigate its suitability for vascular implant applications. The cellular responses of human endothelial progenitor cells (EPCs) to both bare and nitrided Ti-27Nb alloy have been analyzed using Live/Dead staining, MTT assay, fluorescence microscopy and ELISA technique, as well as NiTi alloy for comparison. Live/Dead staining and MTT assay were performed to assess the cellular viability and proliferation, while fluorescence microscopy was used to analyze cell adhesion, cell morphology, and the expression of endothelial cell markers (VE-cadherin and von Willebrand factor). Secretion of the pro-inflammatory chemokine MCP (monocyte chemoattractant protein)-1 by the cells grown in contact with the analyzed materials was further verified using the enzyme-linked immunosorbent assay. The results obtained revealed that adhesion, spreading, viability, proliferation rate and phenotypic markers expression of EPCs were similar on the surfaces of Ti-27Nb and NiTi alloys. Cells exposed to nitrided Ti-27Nb surface exhibited significantly decreased inflammatory response, which may be beneficial for reducing in-stent restenosis incidence.
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