Reassembling green fluorescent protein for in vitro evaluation of trans-translation

Charlotte Guyomar (1) , Marion Thepaut (1, 2) , Sylvie Nonin-Lecomte (3) , Agnes Mereau (1) , Renan Goude (1) , Reynald Gillet (1)

1 IGDR - Institut de Génétique et Développement de Rennes
2 SATT OUEST VALORISATION - Société d'Accélération du Transfert de Technologies
3 CiTCoM - UMR 8038 - Cibles Thérapeutiques et conception de médicaments

In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) in bacteria. This system is based on an engineered tmRNA variant that reassembles the green fluorescent protein (GFP) when trans-translation is active. Our system is adapted for high-throughput screening of chemical compounds by fluorescence.

Domaines: Sciences du Vivant [q-bio]

Charlotte Guyomar, Marion Thepaut, Sylvie Nonin-Lecomte, Agnes Mereau, Renan Goude, et al.. Reassembling green fluorescent protein for in vitro evaluation of trans-translation. Nucleic Acids Research, 2020, 48 (4), pp.e22-e22. ⟨10.1093/nar/gkz1204⟩. ⟨hal-02440937⟩