Identification, partial purification and characterization of high-molecular-weight gelatin-degrading metalloproteinases produced by a rat mammary carcinoma cell line

Wahida Kihal-Talantikite (1) , Séverine Deguen (2) , Cindy Padilla (2) , Muriel Siebert (3) , Cécile Couchoud (4) , Sahar Bayat (1) , N. Pallet , A.-S. Jannot , M. El Bahri , I. Etienne , Matthias Buchler (5) , B. de Ligny , Gabriel Choukroun (6) , C. Colosio , A. Thierry (7) , Cécile Vigneau (8) , B. Moulin , Y. Le Meur (9) , A.-E. Heng (10) , J.-F. Subra (11) , C. Legendre (12) , P. Beaune (13) , C. Alberti (14) , M. Loriot , E. Thervet (15) , Andrew Nethery , Robert O'Grady

1 EHESP - École des Hautes Études en Santé Publique [EHESP]
2 Irset - Institut de recherche en santé, environnement et travail
3 Service de néphrologie [Rennes]
4 Agence de la biomédecine [Saint-Denis la Plaine]
5 Service de néphrologie et immunologie clinique [CHRU Tours]
6 Mécanismes physiologiques et conséquences des calcifications cardiovasculaires: rôle des remodelages cardiovasculaires et osseux
7 CHU Poitiers - Centre hospitalier universitaire de Poitiers
8 CHU Pontchaillou [Rennes]
9 CHU - BREST - Nephrologie - CHRU Brest - Service de Nephrologie
10 Service de Néphrologie - Hémodialyses [CHU Clermont-Ferrand]
11 Service de Néphrologie [Angers]
12 LDO - Domaines Océaniques
13 aucun
14 Unité d'Epidémiologie Clinique
15 Service de Transplantation Rénale

BC1 rat mammary carcinoma cells were found to secrete a unique profile of metalloproteinases, distinguished by two gelatin-degrading metalloproteinases of Mr greater than 220.10(3) and Mr much greater than 220.10(3). These enzymes were each partially purified by gel-filtration chromatography, and inhibitor studies showed them to be metalloproteinases. Under conditions where denatured collagen types I, II, and V were completely degraded, native collagen types I, II, IV and V, fibronectin, fibrinogen, C1q, casein, and denatured transferrin were not degraded significantly by these enzymes. The relationship of these enzymes to other extracellular matrix-degrading metalloproteinases and their possible significance in tumour invasion and metastasis is discussed.

Keywords: ESRD incidence; fine geographic scale; neighbourhood deprivation; spatial analysis; Clinical research; immune modulation; immunosuppressant; calcineurin inhibitor: tacrolimus; pharmacokinetics; nephrology; immunosuppression; pharmacodynamics; genetics; pharmacology; kidney transplantation; practice

Domains: Life Sciences [q-bio]; Human health and pathology

Wahida Kihal-Talantikite, Séverine Deguen, Cindy Padilla, Muriel Siebert, Cécile Couchoud, et al.. Identification, partial purification and characterization of high-molecular-weight gelatin-degrading metalloproteinases produced by a rat mammary carcinoma cell line. Biochimica et Biophysica Acta (BBA) - General Subjects, 1989, 993 (1), pp.42-47. ⟨10.1016/0304-4165(89)90140-2⟩. ⟨hal-02445620⟩