Skip to Main content Skip to Navigation
Journal articles

Characterization of Glyceollins as Novel Aryl Hydrocarbon Receptor Ligands and Their Role in Cell Migration

Abstract : Recent studies strongly support the use of the aryl hydrocarbon receptor (AhR) as a therapeutic target in breast cancer. Glyceollins, a group of soybean phytoalexins, are known to exert therapeutic effects in chronic human diseases and also in cancer. To investigate the interaction between glyceollin I (GI), glyceollin II (GII) and AhR, a computational docking analysis, luciferase assays, immunofluorescence and transcriptome analyses were performed with different cancer cell lines. The docking experiments predicted that GI and GII can enter into the AhR binding pocket, but their interactions with the amino acids of the binding site differ, in part, from those interacting with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Both GI and GII were able to weakly and partially activate AhR, with GII being more potent. The results from the transcriptome assays showed that approximately 10% of the genes regulated by TCDD were also modified by both GI and GII, which could have either antagonistic or synergistic effects upon TCDD activation. In addition, we report here, on the basis of phenotype, that GI and GII inhibit the migration of triple-negative (ER-, PgR-, HER2NEU-) MDA-MB-231 breast cancer cells, and that they inhibit the expression of genes which code for important regulators of cell migration and invasion in cancer tissues. In conclusion, GI and GII are AhR ligands that should be further investigated to determine their usefulness in cancer treatments.
Complete list of metadatas

Cited literature [60 references]  Display  Hide  Download

https://hal-univ-rennes1.archives-ouvertes.fr/hal-02499347
Contributor : Laurent Jonchère <>
Submitted on : Thursday, March 5, 2020 - 10:58:09 AM
Last modification on : Wednesday, June 3, 2020 - 11:10:04 AM
Long-term archiving on: : Saturday, June 6, 2020 - 1:53:31 PM

File

ijms-21-01368-v2.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Thu Ha Pham, Sylvain Lecomte, Remy Le Guevel, Aurélie Lardenois, Bertrand Evrard, et al.. Characterization of Glyceollins as Novel Aryl Hydrocarbon Receptor Ligands and Their Role in Cell Migration. International Journal of Molecular Sciences, MDPI, 2020, 21 (4), pp.1368. ⟨10.3390/ijms21041368⟩. ⟨hal-02499347⟩

Share

Metrics

Record views

335

Files downloads

881