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Journal Articles Food Hydrocolloids Year : 2020

Structural characterization of heat-induced protein aggregates in model infant milk formulas

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Abstract

Heat treatments induce structural modifications of bovine milk proteins. In this study, we aimed to investigate how these structural modifications are affected by the whey protein profile of infant milk formulas (IMFs) and heat treatment parameters. Three model IMFs (1.3% of proteins; caseinwhey protein ratio 4060) differing by the whey protein profile (13.37 α-lactalbumin (α-LA)β-lactoglobulin (β-LG), 13.261.80 α-LAβ-LGlactoferrin (LF) or 10.130.40 α-LAβ-LGLF), were heated at 67.5 °C or 80 °C to reach an akin whey protein denaturation extent of 65%. Protein structures were analyzed by asymmetrical flow field-flow fractionation coupled with multiangle light scattering and differential refractometer, transmission electron microscopy and electrophoresis. The unheated IMFs were used as reference. The results showed that LF addition in IMFs induced partial casein micelle disintegration before heating. In the absence of added LF, the heat-denatured whey proteins either formed soluble whey protein aggregates or casein micelle-bound whey protein aggregates. The latter were favored at the expense of soluble aggregates in the heated IMFs with the LF content increase and the concomitant β-LG content decrease. Consequently, the casein micelle structure was strongly dependent on the β-LG and LF amounts in IMFs and on the heating temperature. In the IMFs containing β-LG and LF and at temperature greater than the β-LG denaturation temperature, the casein micelles exhibited filamentous appendages on its surface after heating. Below the denaturation temperature of β-LG and in IMFs containing trace β-LG amount, the heated casein micelles were perfectly spherical with a smooth surface. © 2020 Elsevier Ltd

Dates and versions

hal-02570394 , version 1 (12-05-2020)

Licence

Attribution - NonCommercial - NoDerivatives - CC BY 4.0

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Cite

A. Halabi, Amélie Deglaire, M. Hennetier, F. Violleau, A. Burel, et al.. Structural characterization of heat-induced protein aggregates in model infant milk formulas. Food Hydrocolloids, 2020, 107, pp.105928. ⟨10.1016/j.foodhyd.2020.105928⟩. ⟨hal-02570394⟩
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