Genotoxic stress triggers the activation of IRE1α-dependent RNA decay to modulate the DNA damage response

Estefanie Dufey; José Manuel Bravo-San Pedro; Cristian Eggers; Matías González-Quiroz; Hery Urra; Alfredo I Sagredo; Denisse Sepulveda; Philippe Pihán; Amado Carreras-Sureda; Younis Hazari; Eduardo A Sagredo; Daniela Gutierrez; Cristian Valls; Alexandra Papaioannou; Diego Acosta-Alvear; Gisela Campos; Pedro M Domingos; Remy Pedeux; Eric Chevet; Alejandra Alvarez; Patricio Godoy; Peter Walter; Álvaro Glavic; Guido Kroemer; Claudio Hetz

doi:10.1038/s41467-020-15694-y

Journal articles

Genotoxic stress triggers the activation of IRE1α-dependent RNA decay to modulate the DNA damage response

Estefanie Dufey ¹ José Manuel Bravo-San Pedro ² Cristian Eggers ¹ Matías González-Quiroz ^{1, 3, 4} Hery Urra ¹ Alfredo I Sagredo ¹ Denisse Sepulveda ¹ Philippe Pihán ¹ Amado Carreras-Sureda ¹ Younis Hazari ¹ Eduardo A Sagredo ⁵ Daniela Gutierrez ⁶ Cristian Valls ⁶ Alexandra Papaioannou ^{3, 4} Diego Acosta-Alvear ^{7, 8} Gisela Campos ^{9, 10} Pedro M Domingos ¹¹ Remy Pedeux ^{3, 4} Eric Chevet ^{3, 4} Alejandra Alvarez ⁶ Patricio Godoy ¹⁰ Peter Walter ⁷ Álvaro Glavic ¹ Guido Kroemer ^{2, 12, 13} Claudio Hetz ¹

1 UCHILE - Universidad de Chile = University of Chile [Santiago]

2 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers

3 COSS - Chemistry, Oncogenesis, Stress and Signaling

4 CRLCC - Centre Eugène Marquis

5 Stockholm University

6 UC - Pontificia Universidad Católica de Chile

7 University of the Pacific [San Francisco]

8 UCSB - University of California [Santa Barbara]

9 TU - Technische Universität Dortmund [Dortmund]

10 IFADO - Leibniz Research Centre for Working Environment and Human Factors [Dortmund]

11 NOVA - Universidade Nova de Lisboa = NOVA University of Lisboa

12 Pôle de biologie

13 Karolinska Institutet [Stockholm]

Abstract : The molecular connections between homeostatic systems that maintain both genome integrity and proteostasis are poorly understood. Here we identify the selective activation of the unfolded protein response transducer IRE1α under genotoxic stress to modulate repair programs and sustain cell survival. DNA damage engages IRE1α signaling in the absence of an endoplasmic reticulum (ER) stress signature, leading to the exclusive activation of regulated IRE1α-dependent decay (RIDD) without activating its canonical output mediated by the transcription factor XBP1. IRE1α endoribonuclease activity controls the stability of mRNAs involved in the DNA damage response, impacting DNA repair, cell cycle arrest and apoptosis. The activation of the c-Abl kinase by DNA damage triggers the oligomerization of IRE1α to catalyze RIDD. The protective role of IRE1α under genotoxic stress is conserved in fly and mouse. Altogether, our results uncover an important intersection between the molecular pathways that sustain genome stability and proteostasis.

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Cancer

Complete list of metadatas

https://hal-univ-rennes1.archives-ouvertes.fr/hal-02862260
Contributor : Laurent Jonchère <>
Submitted on : Tuesday, June 9, 2020 - 1:43:44 PM
Last modification on : Thursday, January 14, 2021 - 11:29:46 AM

qt4789w8jr.pdf?t=qaxr0i

Publisher files allowed on an open archive

Genotoxic stress triggers the activation of IRE1α-dependent RNA decay to modulate the DNA damage response

File

Licence

Identifiers

Collections

Citation

Export

Metrics

54

55

Genotoxic stress triggers the activation of IRE1α-dependent RNA decay to modulate the DNA damage response

File

Licence

Identifiers

Collections

Citation

Export

Share

Metrics

54

55