Communication Between Humans: Towards an Interdisciplinary Model of Intercomprehension
Résumé
Unlabelled - Final fruit size of apple () cultivars is related to both mesocarp cell division and cell expansion during fruit growth, but it is unclear whether the cell division and/or cell enlargement determine most of the differences in fruit size between species. In this study, by using an interspecific hybrid population between "Zisai Pearl" and cultivar "Red Fuji," we found that the mesocarp cell number was the main causal factor of diversity in fruit size between species. Rapid increase in mesocarp cell number occurred prior to 28 days after anthesis (DAA), while cell size increased gradually after 28 DAA until fruit ripening. Six candidate genes related to auxin signaling or cell cycle were predicted by combining the RNA-seq data and previous QTL data for fruit weight. Two InDels and 10 SNPs in the promoter of a small auxin upregulated RNA gene in Zisai Pearl led to a lower promoter activity than that of Red Fuji. One non-synonymous SNP G/T at 379 bp downstream of the ATG codon of , which was heterozygous in Zisai Pearl, exerted significant genotype effects on fruit weight, length, and width. Transgenic apple calli by over-expressing or RNAi confirmed that participated in the negative regulation of mesocarp cell division and thus apple fruit size. These results could provide new insights in the molecular mechanism of small fruit size in accession and be potentially used in molecular assisted breeding via interspecific hybridization. Supplementary information - The online version contains supplementary material available at 10.1007/s11032-024-01441-4.