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Cofilin regulates actin network homeostasis and microvilli length in mouse oocytes

Abstract : How multiple actin networks coexist in a common cytoplasm, while competing for a shared pool of monomers, is still an ongoing question. This is exemplified by meiotic maturation in the mouse oocyte, which relies on the dynamic remodeling of distinct cortical and cytoplasmic F-actin networks. Here we show that the conserved actin-depolymerizing factor cofilin is activated in a switch like manner at meiosis resumption from prophase arrest. Interfering with cofilin activation during maturation resulted in widespread microvilli elongation, while cytoplasmic F-actin was depleted, leading to defects in spindle migration and polar body extrusion. In contrast, cofilin inactivation in metaphase II-arrested oocytes resulted in a shutdown of F-actin dynamics, along with dramatic overgrowth of the polarized actin cap. However, inhibition of the Arp2/3 complex to promote actin cap disassembly elicited ectopic microvilli outgrowth in the polarized cortex. These data establish cofilin as a key player in actin network homeostasis in oocytes, and reveal that microvilli can act as a sink for monomers upon disassembly of a competing network.
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Preprints, Working Papers, ...
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Contributor : Guillaume Halet Connect in order to contact the contributor
Submitted on : Tuesday, October 19, 2021 - 5:44:55 PM
Last modification on : Thursday, November 25, 2021 - 5:30:01 PM

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Guillaume Halet, Anne Bourdais, Benoit Dehapiot. Cofilin regulates actin network homeostasis and microvilli length in mouse oocytes. 2021. ⟨hal-03386275⟩



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