Identification of specific protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Methods in Molecular Biology Année : 2010

Identification of specific protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family.

Résumé

In order to determine how gene expression is regulated in response to environmental cues, it is necessary to identify the specific interaction between transcription factors and their cognate cis-regulatory DNA elements. Here we have out-lined electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) protocols to define in vitro and in vivo USFs specific interacting sequences. The proposed procedures have been optimized for the USFs transcription factor family, allowing the identification of USF-specific targets.
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Dates et versions

inserm-00511968 , version 1 (26-08-2010)

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Marie-Dominique Galibert, Yorann Baron. Identification of specific protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family.. Methods in Molecular Biology, 2010, 647, pp.391-406. ⟨10.1007/978-1-60761-738-9_24⟩. ⟨inserm-00511968⟩
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