Decoding in the absence of a codon by tmRNA and SmpB in the ribosome. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Science Année : 2012

Decoding in the absence of a codon by tmRNA and SmpB in the ribosome.

Résumé

In bacteria, ribosomes stalled at the end of truncated messages are rescued by transfer-messenger RNA (tmRNA), a bifunctional molecule that acts as both a transfer RNA (tRNA) and a messenger RNA (mRNA), and SmpB, a small protein that works in concert with tmRNA. Here, we present the crystal structure of a tmRNA fragment, SmpB and elongation factor Tu bound to the ribosome at 3.2 angstroms resolution. The structure shows how SmpB plays the role of both the anticodon loop of tRNA and portions of mRNA to facilitate decoding in the absence of an mRNA codon in the A site of the ribosome and explains why the tmRNA-SmpB system does not interfere with normal translation.

Dates et versions

inserm-00683297 , version 1 (28-03-2012)

Identifiants

Citer

Cajetan Neubauer, Reynald Gillet, Ann C Kelley, Venkat Ramakrishnan. Decoding in the absence of a codon by tmRNA and SmpB in the ribosome.. Science, 2012, 335 (6074), pp.1366-9. ⟨10.1126/science.1217039⟩. ⟨inserm-00683297⟩
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