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Label-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique

Abstract : We report how a recently developed polarization imaging technique, implementing micro-wave photonics and referred to as orthogonality-breaking (OB) imaging, can be adapted on a classical confocal fluorescence microscope, and is able to provide informative polarization images from a single scan of the cell sample. For instance, the comparison of the images of various cell lines at different cell-cycle stages obtained by OB polarization microscopy and fluorescence confocal images shows that an endogenous polarimetric contrast arizes with this instrument on compacted chromosomes during cell division.
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https://hal.archives-ouvertes.fr/hal-03335552
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Monday, October 25, 2021 - 4:29:52 PM
Last modification on : Monday, October 25, 2021 - 4:29:55 PM

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Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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R. Desapogu, Gilles Le Marchand, R. Smith, P. Ray, É. Gillier, et al.. Label-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique. Biomedical optics express, Optical Society of America - OSA Publishing, 2021, 12 (8), pp.5290-5304. ⟨10.1364/BOE.426630⟩. ⟨hal-03335552⟩

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