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Mechanical Control of Cell Migration by the Metastasis Suppressor Tetraspanin CD82/KAI1

Abstract : The plasma membrane is a key actor of cell migration. For instance, its tension controls persistent cell migration and cell surface caveolae integrity. Then, caveolae constituents such as caveolin-1 can initiate a mechanotransduction loop that involves actin- and focal adhesion-dependent control of the mechanosensor YAP to finely tune cell migration. Tetraspanin CD82 (also named KAI-1) is an integral membrane protein and a metastasis suppressor. Its expression is lost in many cancers including breast cancer. It is a strong inhibitor of cell migration by a little-known mechanism. We demonstrated here that CD82 controls persistent 2D migration of EGF-induced single cells, stress fibers and focal adhesion sizes and dynamics. Mechanistically, we found that CD82 regulates membrane tension, cell surface caveolae abundance and YAP nuclear translocation in a caveolin-1-dependent manner. Altogether, our data show that CD82 controls 2D cell migration using membrane-driven mechanics involving caveolin and the YAP pathway.
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https://hal.archives-ouvertes.fr/hal-03415288
Contributor : Pierre-Emmanuel Milhiet Connect in order to contact the contributor
Submitted on : Tuesday, November 9, 2021 - 2:22:41 PM
Last modification on : Tuesday, December 7, 2021 - 2:24:05 PM

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Laura Ordas, Luca Costa, Anthony Lozano, Christopher Chevillard, Alexia Calovoulos, et al.. Mechanical Control of Cell Migration by the Metastasis Suppressor Tetraspanin CD82/KAI1. Cells, MDPI, 2021, 10 (6), pp.1545. ⟨10.3390/cells10061545⟩. ⟨hal-03415288⟩

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