A general mathematical model for the in vitro assembly dynamics of intermediate filament proteins

Norbert Mücke (1) , Tomasz Wocjan (1) , Marine Jacquier (2) , Harald Herrmann (3) , Stéphanie Portet (4)

1 DKFZ - German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg]
2 IGDR - Institut de Génétique et Développement de Rennes
3 Universitätsklinikum Erlangen [Erlangen]
4 University of Manitoba [Winnipeg]

Intermediate filament (IF) proteins assemble into highly flexible filaments that organize into complex cytoplasmic networks: keratins in all types of epithelia, vimentin in endothelia and desmin in muscle. Since IF elongation proceeds via end-to-end annealing of unit-length filaments and successively of progressively growing filaments, it is important to know, how their remarkable flexibility, i.e., their persistence length l(p), influences the assembly kinetics. In fact, their l(p) ranges between 0.3 μm (keratin K8/K18) and 1.0 μm (vimentin and desmin), and thus is orders of magnitude lower than that of microtubules and F-actin. Here, we present a unique mathematical model, which implements the semiflexible nature of the three IF types based on published semiflexible polymers theories and depends on a single free parameter k(0). Calibrating this model to filament mean length dynamics of the three proteins, we demonstrate that the persistence length is indeed essential to accurately describe their assembly kinetics. Furthermore, we reveal that the difference in flexibility alone does not explain the significantly faster assembly rate of keratin filaments compared to that of vimentin. Likewise, desmin assembles ∼ six times faster than vimentin, even though both their filaments exhibit the same l(p) value. These data strongly indicate that differences in their individual amino acid sequences significantly impact the assembly rates. Nevertheless, using a single k(0) value for each of these three key representatives of the IF protein family, our advanced model does accurately describe the length distribution and mean length dynamics and provides effective filament assembly rates. It thus provides a tool for future investigations on the impact of post-translational modifications or amino acid changes of IF proteins on assembly kinetics. This is an important issue, as the discovery of mutations in IF genes causing severe human disease, particularly for desmin and keratins, is steadily increasing.

Domaines: Sciences du Vivant [q-bio]; Génétique

Norbert Mücke, Tomasz Wocjan, Marine Jacquier, Harald Herrmann, Stéphanie Portet. A general mathematical model for the in vitro assembly dynamics of intermediate filament proteins. Biophysical Journal, 2022, 121 (6), pp.1094-1104. ⟨10.1016/j.bpj.2022.02.001⟩. ⟨hal-03578492⟩