Skip to Main content Skip to Navigation
Journal articles

In vitro assessment of enzymatic phytate dephosphorylation during digestive process of different feeds and feed ingredients

Abstract : In vivo studies of the digestive process are long, expensive and difficult to rationalize, whereas in vitro systems may give more accessible insight into parts of this process. The purpose of this study was to show the ability of a three-step simulation of monogastric animals' digestive system to estimate phytate hydrolysis and how it is affected by feed composition. Several feed ingredients: wheat, maize, soybean meal and rapeseed meal and complete diets: a wheat-maize-soybean-mealbased diet, a maize-soybean-meal diet and a wheat-maize-rapeseed-meal diet were treated using an adaptation of a described in vitro digestion simulation system in the presence of increasing doses of phytase. A strong dependence of phytate hydrolysis on the feed ingredient used was obtained: phosphorus releases were 0.3, 0.8, 1.0 and 1.6 g/kg at 0 U/kg of phytase supplementation for maize, soybean meal, wheat and rapeseed meal respectively and 1.2, 2.9, 1.7 and 3.9 at 1000 U/kg of bacterial phytase. The efficacy of enzymatic dephosphorylation of phytate was found dependent on the ingredient, which can be partially explained by their initial content in myo-inositol phosphates. The in vitro simulation was proven a useful tool to assess enzymatic dephosphorylation of phytate under different conditions.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03413929
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Tuesday, November 23, 2021 - 1:51:46 PM
Last modification on : Tuesday, January 4, 2022 - 6:40:38 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2022-03-30

Please log in to resquest access to the document

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Citation

A. Riviere, T. Nothof, R. Greiner, Sylvain Tranchimand, Nicolas Noiret, et al.. In vitro assessment of enzymatic phytate dephosphorylation during digestive process of different feeds and feed ingredients. Animal Feed Science and Technology, Elsevier Masson, 2021, 281, pp.115096. ⟨10.1016/j.anifeedsci.2021.115096⟩. ⟨hal-03413929⟩

Share

Metrics

Les métriques sont temporairement indisponibles