Structural origin of the drastic modification of second harmonic generation intensity pattern occurring in tail muscles of climax stages xenopus tadpoles.

Gaëlle Recher (1, 2) , Pascal Coumailleau (1) , Denis Rouède (3) , François Tiaho (1)

1 Irset - Institut de recherche en santé, environnement et travail
2 Dept of Physiology, Development and Neurosciences, Anatomy building, University of Cambridge
3 IPR - Institut de Physique de Rennes

Second harmonic generation (SHG) microscopy is a powerful tool for studying submicron architecture of muscles tissues. Using this technique, we show that the canonical single frequency sarcomeric SHG intensity pattern (SHG-IP) of premetamorphic xenopus tadpole tail muscles is converted to double frequency (2f) sarcomeric SHG-IP in metamorphic climax stages due to massive physiological muscle proteolysis. This conversion was found to rise from 7% in premetamorphic muscles to about 97% in fragmented muscular apoptotic bodies. Moreover a 66% conversion was also found in non-fragmented metamorphic tail muscles. Also, a strong correlation between predominant 2f sarcomeric SHG-IPs and myofibrillar misalignment is established with electron microscopy. Experimental and theoretical results demonstrate the higher sensitivity and the supra resolution power of SHG microscopy over TPEF to reveal 3D myofibrillar misalignment. From this study, we suggest that 2f sarcomeric SHG-IP could be used as signature of triad defect and disruption of excitation–contraction coupling. As the mechanism of muscle proteolysis is similar to that found in mdx mouse muscles, we further suggest that xenopus tadpole tail resorption at climax stages could be used as an alternative or complementary model of Duchene muscular dystrophy.

Keywords: Skeletal muscle; Second harmonic generation microscopy; Two photon excitation fluorescence; Electron microscopy; Xenopus; Apoptosis

Domains: Life Sciences [q-bio]

Gaëlle Recher, Pascal Coumailleau, Denis Rouède, François Tiaho. Structural origin of the drastic modification of second harmonic generation intensity pattern occurring in tail muscles of climax stages xenopus tadpoles.. Journal of Structural Biology, 2015, 190 (1), pp.1-10. ⟨10.1016/j.jsb.2015.03.003⟩. ⟨hal-01134174⟩