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Isolation of Bioactive Compounds from Calicotome villosa Stems

Abstract : A phenylethanoid, two steroids, a flavone glucoside and a chalcone have been isolated for the first time from the stems of together with a previously isolated flavone glucoside. Their structures were determined by spectroscopic analyses (NMR, HRMS) as basalethanoïd B (), β-sitosterol and stigmasterol (), chrysine-7--β-d-glucopyranoside (), chrysine 7-((6''--acetyl)--β-d-glucopyranoside) () and calythropsin (). The crude extracts and the isolated compounds (except 4), were evaluated for their antioxidant, antimicrobial (against two Gram-positive bacterial strains: , , four Gram-negative bacterial strains: , , , and three yeasts: , , and ), hemolytic, antidiabetic, anti-inflammatory and cytotoxic activity. The crude extracts showed good ability to scavenge the free radical DPPH. Methanol stem extract followed by the dichloromethane stem extract showed moderate antimicrobial potency; furthermore, at 1 mg/mL the methanol extract showed an inhibition of growth comparable to nystatin. Dichloromethane, methanol, and aqueous extracts inhibited 98%, 90%, and 80% of HeLa cell proliferation at 2 mg/mL respectively. Weak hypoglycemic and hemolytic effects were exhibited by the crude extracts. Among all the tested compounds, compound showed remarkable hypoglycemic potential (93% at 0.1 mg/mL) followed by compound (90% at 0.3 mg/mL). Compound was the most effective in the DPPH scavenging assay (100% at 0.1 mg/mL) and cytotoxic assay on HeLa cells (99% and 90% after 24 and 48 h of treatment at 0.1 mg/mL, respectively). No anti-inflammatory effects were displayed by any of the crude extracts or the isolated compounds at any of the tested concentrations.
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Submitted on : Monday, April 23, 2018 - 3:47:47 PM
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J. Alhage, H. Elbitar, S. Taha, J.-P. Guegan, Z. Dassouki, et al.. Isolation of Bioactive Compounds from Calicotome villosa Stems. Molecules, MDPI, 2018, 23 (4), pp.851. ⟨10.3390/molecules23040851⟩. ⟨hal-01774427⟩

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