 |
 |
 |
Journal articles
Reassembling green fluorescent protein for in vitro evaluation of trans-translation
Abstract : In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) in bacteria. This system is based on an engineered tmRNA variant that reassembles the green fluorescent protein (GFP) when trans-translation is active. Our system is adapted for high-throughput screening of chemical compounds by fluorescence.
Cited literature [34 references]
https://hal-univ-rennes1.archives-ouvertes.fr/hal-02440937
Contributor : Laurent Jonchère <>
Submitted on : Friday, July 17, 2020 - 10:05:34 AM
Last modification on : Thursday, January 14, 2021 - 11:19:59 AM
Long-term archiving on: : Monday, November 30, 2020 - 11:51:02 PM
Contributor : Laurent Jonchère <>
Submitted on : Friday, July 17, 2020 - 10:05:34 AM
Last modification on : Thursday, January 14, 2021 - 11:19:59 AM
Long-term archiving on: : Monday, November 30, 2020 - 11:51:02 PM
File
gkz1204.pdf
Publication funded by an institution
Licence
Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License