Sub-chronic exposure to Kalach 360 SL, Glyphosate-based Herbicide, induced bone rarefaction in female Wistar rats

Latifa Hamdaoui (1) , Hassane Oudadesse (2) , Bertrand Lefeuvre (2) , Asma Mahmoud (1) , Manel Naifer (3) , Riadh Badraoui (1) , Fatma Ayadi (3) , Tarek Rebai (1)

1 Université de Sfax - University of Sfax
2 ISCR - Institut des Sciences Chimiques de Rennes
3 Hopital Habib Bourguiba - Habib Bourguiba Hospital [Sfax]

We investigated the effects of Kalach 360 SL (KL), Glyphosate (G)-based herbicide, on bone tissue in different groups of female Wistar rats. Group 1 (n = 6) received a standard diet and served as a control, groups 2 and 3 (n = 6 each) received 0.07 ml (D1 126 mg/Kg) and 0.175 ml (D2 315 mg/Kg) of KL dissolved in the water for 60 days. The plasma was used to examine the metabolic balance markers (calcium, phosphorus, phosphatase alkaline (PAL), and vitamin D (vit D) and hormonal status (oestrogen and thyroid hormones). As a result, sub-chronic exposure to KL induced a perturbation of bone metabolism (calcium and phosphorus) and hormonal status disturbance. The histological and immunohistochemical study of the thyroid gland revealed a disturbance in morphological structure and thyroid cells function. Moreover, the KL disrupting eff ;ect on thyroid function was investigated by measuring changes in plasma levels of thyroid hormones. Free triiodothyronine (FT3) and thyroxine (FT4) were decreased in female rats breast-fed from rats treated with D and D2 of KL. This eff ;ect was associated with an increase in the plasma level of thyroid-stimulating hormone (TSH). Thus, that KL leads to hypothyroidism. Decrease in levels of oestrogen and thyroid dysfunction led to a disruption in the skeletal bone. The histological study and SEM in bone results allowed us to observe, in rats exposed to KL, the thinning and discontinuity of bone trabecular with a significant decrease in the number of nodes (intertrabecular links).In conclusion, KL sub-chronic exposure caused an aspect of osteoporosis.

Mots clés: Glyphosate; Kalach 360 SL; Osteoporosis; Rats; Bone tissue; Bone remodeling

Domaines: Toxicologie

Latifa Hamdaoui, Hassane Oudadesse, Bertrand Lefeuvre, Asma Mahmoud, Manel Naifer, et al.. Sub-chronic exposure to Kalach 360 SL, Glyphosate-based Herbicide, induced bone rarefaction in female Wistar rats. Toxicology, 2020, 436, pp.152412. ⟨10.1016/j.tox.2020.152412⟩. ⟨hal-02533168⟩